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KMID : 0545120170270061112
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 6 p.1112 ~ p.1119
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Bioethanol Production from Hydrodictyon reticulatum by Fed-Batch Fermentation Using Saccharomyces cerevisiae KCTC7017
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Kim Seul-Ki
Nguyen Cuong Mai
Ko Eun-Hye
Kim In-Chul
Kim Jin-Seog
Kim Jin-Cheol
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Abstract
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The aim of this study was to develop a potential process for bioethanol production from Hydrodictyon reticulatum (HR), a filamentous freshwater alga, using Saccharomyces cerevisiae (KCTC7017). From the sugar solutions prepared by the four different hydrolysis methods, bioethanol production ranged from 11.0 g/100 g dried material (acid hydrolysis) to 22.3 g/ 100 g dried material (enzymatic hydrolysis, EH). Bioethanol was fermented from a highly concentrated sugar solution obtained by a decompression-mediated (vacuum) enrichment method (VE). As the results, ethanol was more efficiently produced from HR when sugar solutions were concentrated by VE following EH (EH/VE). Using multiple feeding of the sugar solution prepared by EH/VE from HR, ethanol reached up to a concentration of 54.3 g/l, corresponding to 24.9 g/100 g dried material, which attained the economic level of product concentration (approximately 5%). The results indicate that by using HR, it is feasible to establish a bioethanol production process, which is effective for using microalgae as the raw material for ethanol production.
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KEYWORD
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Bioethanol, fermentation, freshwater algae, Hydrodictyon reticulatum, hydrolysis
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